Brand | MultiSciences |
CatNum | 70-EK205 |
Product Name | Mouse IL-5 ELISA Kit |
Customs Name | Mouse IL-5 ELISA Kit |
Application | ELISA |
Reactivity | Mouse |
Assay Type | Sandwich ELISA |
Suitable Sample Type | serum, plasma, cell culture supernates |
Format | 96-well strip plate |
Storage | 4 (unopened) standard stored at -20, others stored at 4 (opened) |
Shipping Condition | 4 |
Sample Volume | 10 l |
Sensitivity | 3.14 pg/ml |
Standard Curve Range | 15.63 - 1000 pg/ml |
Spike Recovery Range | 80 % - 118 % |
Mean Spike Recovery | 1 |
CV of Intra plate | 2.7 % - 4.2 % |
CV of Inter plate | 2.5 % - 4.2 % |
Release Date | 2016/8/20 |
Components | 96-well polystyrene microplate coated with a monoclonal antibody against Dkk-1 Mouse Dkk-1 Standard, lyophilized Dkk-1 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10) Substrate (TMB) Stop Solution Washing Buffer (20) Plate Covers |
Describtion |
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IL-5. The Mouse IL-5 ELISA is for research use only. Not for diagnostic or therapeutic procedures. IL-5 is a 115-amino acid-long Th2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer. IL-5 is produced by Th2 cells and mast cells. The spectrum of biologicalactivities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of Th2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunoglobulin secretion. It is also a key mediator in eosinophil activation. IL-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, wherein a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed. |
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